Video 1

Movie S1. Representative movie of wtDC-SIGN on live CHO cells captured by dual-color SPT. wtDC-SIGN was labeled with biotinylated single-chain DCN46 conjugated with QD585 (green) or QD655 (red). Notice that interactions are brief with no evidence for sustained correlated motion as confirmed by the simulations shown in Fig. 2C. Frame rate, 30 Hz. Total area, 2 × 2 μm2.

Enhanced receptor–clathrin interactions induced by N-glycan–mediated membrane micropatterning

Juan A. Torreno-Pina, Bruno M. Castro, Carlo Manzo, Sonja I. Buschow, Alessandra Cambi, and Maria F. Garcia-Parajo

PNAS. 2014. 111:11037-11042 DOI: 10.1073/pnas.1402041111